ESP Biography

Brief Biographical Sketch:

Phone: (650)723-7439; Email: olgar1@stanford.edu
Stanford University School of Medicine, Department of Radiation Oncology; 269 Campus Drive, CCSR-South #1250, Stanford, CA 94305-5152

EDUCATION:
2006 PhD in Molecular Biology/Cancer Biology,
Engelhardt Institute of Molecular Biology, Moscow, Russia.

2000 MS in Genetics/Molecular Genetics with honors,
Lomonosov Moscow State University, Moscow, Russia.

RESEARCH INTERESTS:
Establishment of the components in the tumor microenvironment affecting tumor gene expression and tumor progression, especially metastasis. Identification of druggable targets to prevent tumor growth and metastatic spread. Identification of genes being synthetically lethal to the loss of known tumor suppressors (like VHL in renal cancer) and dissection of the underlying mechanism.

RESEARCH EXPERIENCE:
2006 – Present Postdoctoral Scholar, Department of Radiation Oncology, Stanford University School of Medicine
(Research Advisor: Dr. Amato J. Giaccia).
Projects: Established the role of CDCP1 protein in renal cancer progression. Conducted the screening and identified the drugs being synthetically lethal to VHL loss in CC-RCC.
2003 – 2006 Research Fellow, Department of Molecular Cardiology, Lerner Research Institute, The Cleveland Clinic
(Research Advisor: Dr. Tatiana V. Byzova).
Projects: Dissected the regulation of outside-in β3 integrin signaling in normal endothelium and cancer. Investigated the consequences of impaired integrin signaling in immortalized lymphocytes of patients, characterized by LAD-III (leukocyte adhesion deficiency) syndrome.
2001 – 2003 Research Fellow, Department of Molecular Genetics, Lerner Research Institute, The Cleveland Clinic
(Research Advisor: Dr. Peter M. Chumakov).
Projects: Assessed the functionality of p53 pathway in melanoma and renal carcinoma cell lines. Developed a series of cell-based readout systems for measuring p53, HIF-1 or HSF-1 transcriptional activity.
2000 – 2003 Graduate student, Engelhardt Institute of Molecular Biology, Moscow, Russia
(Research Advisor: Dr. Peter M. Chumakov).
Dissertation: “Studies of relationships of p53-dependent signal transduction pathway with other pathways of stress response”. Acquired degree (2006) – PhD.
1998 – 2000
Research Fellow, Engelhardt Institute of Molecular Biology, Moscow, Russia
(Research Advisor: Akad. Yuri V. Il’in).
Project: Compared the structure of retrovirus gypsy in different Drosophila melanogaster stocks.
1995 – 2000 Undergraduate student, Department of Genetics, Lomonosov Moscow State University, Moscow, Russia
(Research Advisor: Dr. Alexander I. Kim).
Diploma research: “Studies of Drosophila melanogaster genome instability caused by transpositions of retrovirus gypsy”. Acquired degree (2000) – MS.

TEACHING INTERESTS:
Molecular Biology, Molecular Genetics, Radiation Oncology, Cancer Biology.

TEACHING/MENTORING EXPERIENCE:
2006 – Present Mentor, Department of Radiation Oncology, Stanford University School of Medicine. Trained one undergraduate and three graduate students in molecular biology.
2001 – 2006 Mentor, Departments of Molecular Genetics and Molecular Cardiology, Lerner Research Institute, The Cleveland Clinic. Trained two undergraduate and two graduate students in molecular biology.
2000 Mentor, Engelhardt Institute of Molecular Biology, Moscow, Russia.
Trained two undergraduate students in molecular genetics.

PUBLICATIONS:
1. Razorenova O.V., Finger E., Colavitti R., Chernikova S.B., Boiko A.D., Chan C., Krieg A.J., Bedogni B., LaGory E., Weissman I.L., Broome-Powell M., Giaccia A.J. VHL loss in renal cell carcinoma leads to HIF-dependent upregulation of CUB domain-containing protein 1 to stimulate PKCδ-driven migration. Proc Natl Acad Sci USA. 2011; 108(5):1931-1936.

2. Razorenova O.V. and Giaccia A.J. Hypoxia, Gene Expression, and Metastasis. Chapter 3 in "The Tumor Microenvironment" Book, ed. Bagley RG. 2010; pp 43-58.

3. Boiko A.D., Razorenova O.V., van de Rijn M., Swetter S.M., Johnson D.L., Ly D.P., Butler P.D., Yang G.P., Joshua B., Kaplan M.J., Longaker M.T., Weissman I.L. Human melanoma-initiating cells express neural crest nerve growth factor receptor CD271. Nature. 2010; 466:133-137.

4. Chernikova S.B., Dorth J.A., Razorenova O.V., Game J.C., Brown J.M. Deficiency in Bre1 Impairs Homologous Recombination Repair and Cell Cycle Checkpoint Response to Radiation Damage in Mammalian Cells. Radiat Res. 2010; 174(5):558-65.

5. Krieg A.J., Rankin E.B., Chan D., Razorenova O., Fernandez S., Giaccia A.J. Regulation of the Histone Demethylase JMJD1A by HIF-1α Enhances Hypoxic Gene Expression and Tumor Growth. Mol Cell Biol. 2009; 30(1):344-353.

6. Malinin N.L., Zhang L., Choi J., Ciocea A., Razorenova O., Ma Y.Q., Podrez E.A., Tosi M., Lennon D.P., Caplan A.I., Shurin S.B., Plow E.F., Byzova T.V. A point mutation in KINDLIN3 ablates activation of three integrin subfamilies in humans. Nature Medicine. 2009; 15(3):313-318.

7. Mahabeleshwar G.H., Chen J., Feng W., Somanath P.R., Razorenova O.V., Byzova T.V. Integrin affinity modulation in angiogenesis. Cell Cycle. 2008; 7(3):335-347.

8. Somanath P.R.*, Razorenova O.V.*, Chen J., Byzova T.V. Role for Akt1 kinase in angiogenesis and endothelial cell biology. Cell Cycle. 2006; 5(5):512-518. Review.
* - equal contribution.

9. Boiko A.D., Porteous S., Razorenova O.V., Krivokrysenko V.I., Williams B., Gudkov A.V. A systematic search for downstream mediators of tumor suppressor function of p53 reveals a major role of BTG2 in suppression of Ras-induced transformation. Genes and Development. 2006; 20(2):236-252.

10. Chen J.*, Somanath P.R.*, Razorenova O., Chen W.S., Hay N., Bornstein P., Byzova T.V. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo. Nature Medicine. 2005; 11(11):1188-1196.
* - equal contribution.

11. De S., Razorenova O., McCabe N.P., O’Toole T.E., Qin J., Byzova T.V. VEGF–integrin interplay controls tumor growth and vascularization. Proc Natl Acad Sci USA. 2005; 102(21):7589-7594.

12. Narizhneva N.V., Razorenova O.V., Podrez E.A., Chen J., Chandraesekharan U., DiCorleto P.E., Plow E.F., Topol E.J., Byzova T.V. Thrombospondin-1 upregulates expression of cell adhesion molecules and promotes monocyte binding to endothelium. FASEB J. 2005; 19(9):1158-1160.

13. Razorenova O.V., Ivanov A.V., Budanov A.V., Chumakov P.M. Development of virus-based reporter systems for monitoring transcriptional activity of hypoxia-inducible factor 1. Gene. 2005; 350(1):89-98.

14. Razorenova O.V., Agapova L.S., Chumakov P.М. p53 gene expression and activation of p53-dependent transcription in melanoma cell lines. Mol Biol (Mosk). 2005; 39(3):445-456. Russian.

15. Razorenova O.V., Agapova L.S., Budanov A.V., Ivanov А.V., Strunina S.М., Chumakov P.М. Retroviral reporter systems for the assessment of activity of stress-induced signal transduction pathways controlled by p53, HIF-1 and HSF-1 transcription factors. Mol Biol (Mosk). 2005; 39(2):286-293. Russian.

16. Gurova K.V., Hill J.E., Razorenova O.V., Chumakov P.M., Gudkov A.V. p53 pathway in renal cell carcinoma is repressed by a dominant mechanism. Cancer Res. 2004; 64(6):1951-1958.

17. Razorenova O.V., Karpova N.N., Smirnova Iu.B., Kusulidu L.K., Reneva N.K., Subocheva E.A., Kim A.I., Liubomirskaia N.V., Il'in Iu.V. Interlineage distribution and characteristics of the structure of two subfamilies of Drosophila melanogaster MDG4 (gypsy) retrotransposon. Genetika. 2001; 37(2):175-182. Russian.

18. Lyubomirskaya N.V., Smirnova J.B., Razorenova O.V., Karpova N.N., Surkov S.A., Avedisov S.N., Kim A.I., Ilyin Y.V. Two variants of the Drosophila melanogaster retrotransposon gypsy (MDG4): structural and functional differences, and distribution in fly stocks. Mol Genet Genomics. 2001; 265(2):367-374.

19. Kusulidu L.K., Karpova N.N., Razorenova O.V., Glukhov I.A., Kim A.I., Liubomirskaia N.V., Il'in Iu.V. Mobile genetic element MDG4 (gypsy) in Drosophila melanogaster. Features of structure and regulation of transposition. Genetika. 2001; 37(12):1589-1597. Russian.

20. Kusulidu L.K., Karpova N.N., Razorenova O.V., Glukhov I.A., Kim A.I., Lyubomirskaya N.V., Ilyin Y.V. Structural polymorphism of the transposable element MDG4 (gypsy) in Drosophila melanogaster strains. Doklady Akademii Nauk. 2001; 376(1):133-138. Russian.

21. Kim A.I., Pasiukova E.G., Karpova N.N., Razorenova O.V. Genomic factors regulating the transposition of Drosophila mobile elements. Genetika. 1999; 35(11):1511-1521. Review. Russian.

ORAL PRESENTATIONS:
2010 Program in Radiation Biology Seminar Series. Role of CDCP1 in Clear Cell Renal Cell Carcinoma. Department of Radiation Oncology, Stanford School of Medicine, Stanford, CA.

2006 Molecular Cardiology Seminar Series. Outside-in β3 integrin signaling in endothelium. Department of Molecular Cardiology, Lerner Research Institute, the Cleveland Clinic, Cleveland, OH.

2005 PhD Defense talk. p53-dependent signaling pathways in cancer cells with WT p53. Engelhardt Institute of Molecular Biology, Moscow, Russia.

2005 Molecular Cardiology Seminar Series. Studies of causes and consequences of impaired integrin signaling in immortalized lymphocytes of patients, characterized by LAD-III (leukocyte adhesion deficiency) syndrome. Department of Molecular Cardiology, Lerner Research Institute, the Cleveland Clinic, Cleveland, OH.

2004 Molecular Cardiology Seminar Series. Defining the role of integrins using small interfering RNA technology. Department of Molecular Cardiology, Lerner Research Institute, the Cleveland Clinic, Cleveland, OH.

2000 MS Defense talk. Studies of Drosophila melanogaster genome instability caused by transpositions of retrovirus gypsy. Department of Genetics, Lomonosov Moscow State University, Moscow, Russia.

POSTER PRESENTATIONS:
1. Razorenova O.V., Finger E., Colavitti R., Chernikova S.B., Boiko A.D., Chan C., Krieg A.J., Bedogni B., LaGory E., Weissman I.L., Broome-Powell M., Giaccia A.J. HIF-dependent over-expression of CUB domain-containing protein 1 stimulates migration in clear cell renal cell carcinoma. 102nd AACR Annual Meeting. Orange County Convention Center in Orlando, Florida, April 2-6, 2011. Late-breaking abstracts p.105. Abstract no.LB-357.

2. De S.*, Razorenova O.V.*, McCabe N.P., Byzova T.V. Role of integrin activation in tumor growth and angiogenesis in vivo. An AACR Special Conference in Cancer Research. “Anti-angiogenesis and drug delivery to tumors: bench to bedside and back”. Waltham-Boston, Massachusetts, November 9-13, 2005. Abstract no.B39.
* - equal contribution.

3. Razorenova O.V.*, Narizhneva N.V.*, Somanath P.R., Byzova T.V. Defining the role of integrins using small interfering RNA technology. 10th Biennial Midwest Platelet Conference. Milwaukee, Wisconsin, October 8-10, 2004. Abstract no.P10.
* - equal contribution.

4. Razorenova O.V., Ivanov A.V., Budanov A.V., Byzova T.V., Chumakov P.M. Development of readout system for monitoring HIF-1 transcriptional activity. The Annual Meeting of the American Society for Biochemistry and Molecular Biology and the 8th Conference of the International Union for Biochemistry and Molecular Biology. Boston, Massachusetts, June 12-16, 2004. Late-breaking abstracts p.39. Abstract no.B526.

HONORS:
2000 Honors Diploma and gold medal awarded by Lomonosov Moscow State University, Moscow, Russia.

PROFESSIONAL MEMBERSHIPS:
2006, 2011 Associate member of American Association of Cancer Research.

LANGUAGES:
Russian (native), English (fluent), French (advanced), Italian (basic).

Past Classes